Accedi

Nexcelom Bioscience LLC

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Charlotte Berkes^1,2, Leo Li-Ying Chan^2,3, Alisha Wilkinson^1,2,3, Benjamin Paradis^2,3

¹Department of Biology, Merrimack College, ²Center for Biotechnology and Biomedical Sciences, Merrimack College, ³Department of Technology R&D, Nexcelom Bioscience LLC

Here, we demonstrate how image cytometry can be used for quantification of pathogenic fungi in association with host cells in culture. This technique can be used as an alternative to CFU enumeration.

Cancer Research

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Yongyang Huang¹, Jinyun Zou¹, Mudabbir Badar¹, Junchao Liu¹, Wentao Shi⁵, Shunqiang Wang², Qiongyu Guo³, Xiaofang Wang¹, Sarah Kessel⁴, Leo Li-Ying Chan⁴, Peter Li⁴, Yaling Liu^2,5, Jean Qiu⁴, Chao Zhou^1,5,6

¹Department of Electrical and Computer Engineering, Lehigh University, ²Department of Mechanical Engineering, Lehigh University, ³Department of Biomedical Engineering, Southern University of Science and Technology, ⁴Department of Technology R&D, Nexcelom Bioscience LLC, ⁵Department of Bioengineering, Lehigh University, ⁶Center for Photonics and Nanoelectronics, Lehigh University

Optical coherence tomography (OCT), a three-dimensional imaging technology, was used to monitor and characterize the growth kinetics of multicellular tumor spheroids. Precise volumetric quantification of tumor spheroids using a voxel counting approach, and label-free dead tissue detection in the spheroids based on intrinsic optical attenuation contrast, were demonstrated.