Accedi

University of Witten/Herdecke

2 ARTICLES PUBLISHED IN JoVE

Biology

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

¹Institute for Physiology, Pathophysiology, & Toxicology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke, ²Institute for Immunology & Experimental Oncology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke

Calcium is involved in numerous physiological and pathophysiological signaling pathways. Live cell imaging requires specialized equipment and can be time consuming. A quick, simple method using a flow cytometer to determine relative changes in cytosolic calcium in adherent epithelial cells brought into suspension was optimized.

Bioengineering

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Nadine Rommerswinkel¹, Bernd Niggemann¹, Silvia Keil¹, Kurt S. Zänker¹, Thomas Dittmar¹

¹Institute of Immunology & Experimental Oncology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University

Cell migration is a biological phenomenon that is involved in a plethora of physiological, such as wound healing and immune responses, and pathophysiological processes, like cancer. The 3D-collagen matrix migration assay is a versatile tool to analyze the migratory properties of different cell types within in a 3D physiological-like environment.