Accedi

Jeonbuk National University

2 ARTICLES PUBLISHED IN JoVE

Genetics

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Ju-Chun Chang¹, Se Jin Lee², Jae Su Kim², Chung-Hsiung Wang³, Yu-Shin Nai¹

¹Department of Biotechnology and Animal Science, National Ilan University, ²Department of Agricultural Biology, College of Agriculture Life Sciences, Chonbuk National University, ³Department of Entomology, National Taiwan University

This protocol describes a heat shock-induced protein expression system (pDHsp/V5-His/sf9 cell system), which can be used for either expressing foreign proteins or evaluating the anti-apoptotic activity of potential foreign proteins and their truncated amino acids in insect cells.

Biology

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Yao-Chia Liu¹, Nian-Tong Ni¹, Ju-Chun Chang¹, Yi-Hsuan Li¹, Mi Rong Lee², Jae Su Kim^2,3, Yu-Shin Nai¹

¹Department of Entomology, National Chung Hsing University, ²Department of Agricultural biology, Jeonbuk National University, ³Department of Agricultural Convergence Technology, Jeonbuk National University

Here we present a protocol based on the mealworm (Tenebrio molitor)-bait system that was used for isolating and selecting entomopathogenic fungi (EPF) from soil samples. An effective conidia number (ECN) formula is used to select high stress tolerant EPF based on physiological characteristics for pest microbial control in the field.