Accedi

national institute for basic biology

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Masatake Kanai¹, Shoji Mano^1,2, Mikio Nishimura³

¹Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity, National Institute for Basic Biology, ²Department of Basic Biology, SOKENDAI (The Graduate University for Advanced Studies), ³Department of Cell Biology, National Institute for Basic Biology

We have succeeded in establishing a method for RNA isolation from plant seeds containing large amounts of oils, proteins, and polyphenols, which have inhibitory effects on high-purity RNA isolation. Our method is suitable for monitoring the expression of genes with low level transcripts in seeds.

Developmental Biology

Injecting Gryllus bimaculatus Eggs

Samantha K. Barry¹, Taro Nakamura², Yuji Matsuoka³, Christoph Straub⁴, Hadley W. Horch⁴, Cassandra G. Extavour⁵

¹Colby College, ²Division of Evolutionary Developmental Biology, National Institute for Basic Biology, ³Department of Biological Sciences, National University of Singapore, ⁴Department Biology and Department of Neuroscience, Bowdoin College, ⁵Department of Organismic and Evolutionary Biology and Department of Molecular and Cellular Biology, Harvard University

Here we present a protocol to inject cricket eggs, a technique which serves as a foundational method in many experiments in the cricket, including, but not limited to, RNA interference and genomic manipulation.