national institute for basic biology
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Masatake Kanai 1, Shoji Mano 1,2, Mikio Nishimura 3
1Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity, National Institute for Basic Biology, 2Department of Basic Biology, SOKENDAI (The Graduate University for Advanced Studies), 3Department of Cell Biology, National Institute for Basic Biology
We have succeeded in establishing a method for RNA isolation from plant seeds containing large amounts of oils, proteins, and polyphenols, which have inhibitory effects on high-purity RNA isolation. Our method is suitable for monitoring the expression of genes with low level transcripts in seeds.
Developmental Biology
Injecting Gryllus bimaculatus Eggs
Samantha K. Barry 1, Taro Nakamura 2, Yuji Matsuoka 3, Christoph Straub 4, Hadley W. Horch 4, Cassandra G. Extavour 5
1Colby College, 2Division of Evolutionary Developmental Biology, National Institute for Basic Biology, 3Department of Biological Sciences, National University of Singapore, 4Department Biology and Department of Neuroscience, Bowdoin College, 5Department of Organismic and Evolutionary Biology and Department of Molecular and Cellular Biology, Harvard University
Here we present a protocol to inject cricket eggs, a technique which serves as a foundational method in many experiments in the cricket, including, but not limited to, RNA interference and genomic manipulation.
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