Accedi

Instituto de Investigaciones Biológicas Clemente Estable (IIBCE)

2 ARTICLES PUBLISHED IN JoVE

Biology

Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions

Rosana Rodríguez-Casuriaga¹, Gustavo A. Folle², Federico Santiñaque², Beatriz López-Carro², Adriana Geisinger^1,3

¹Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), ²Servicio de Citometría de Flujo y Clasificación Celular (SECIF), Instituto de Investigaciones Biológicas Clemente Estable, ³Sección Bioquímica, Facultad de Ciencias, Universidad de la República

A novel protocol for the mechanical preparation of testicular cell suspensions from rodent material, avoiding enzymes and detergents, is described. The method is very simple, fast, reproducible, and renders good quality cell suspensions, which are suitable for flow sorting and RNA extraction.

Developmental Biology

Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots

Ines Rauschert^*2, Juan Claudio Benech^*2, Martha Sainz¹, Omar Borsani¹, Mariana Sotelo-Silveira¹

¹Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República (UdelaR), ²Laboratorio de Señalización Celular y Nanobiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE)

The atomic force microscopy indentation protocol offers the possibility to dissect the role of the physical properties of the cell wall of a particular cell of a tissue or organ during normal or constrained growth (i.e., under water deficit).