Accedi

Yantai University

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

Jennifer M. Brazill¹, Yi Zhu¹, Chong Li¹, R. Grace Zhai^1,2

¹Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, ²School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University

We have developed a simple and adaptable workflow to extract quantitative data from fluorescence-imaging-based cell biological studies of protein aggregation and autophagic flux in the central nervous system of Drosophila models of neurodegeneration.

Neuroscience

A Rat Model of Middle Cerebral Artery Occlusion/Reperfusion without Damaging the Anatomical Structure of Cerebral Vessels

Zhang Jiren¹, Jia Xue², Sun Yufei², Li Huiwen², Yang Jian¹, Qi Shilei¹, Zhao Dongman¹, Xie Lei¹, Jia Mingming¹, Wang Tian², Zhang Pinyuan¹

¹Department of Neurosurgery, The Third Hospital of Hebei Medical University, ²School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University

The current protocol describes a rat model of middle cerebral artery occlusion/reperfusion that preserves the anatomical structure of cerebral vessels without causing damage.