Accedi

Chinese Center For Disease Control And Prevention

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1^-/- Mice

Yongli Zhang^*1,2, Hangjie Zhang^*2, Wenqiang Ma^*3, Kefang Liu^1,2, Min Zhao⁴, Yingze Zhao², Xuancheng Lu⁵, Fuping Zhang⁶, Xiangdong Li³, George F. Gao^1,2,4,6, William J. Liu^1,2

¹School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, ²NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, ³State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, ⁴Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, ⁵Laboratory Animal Center, Chinese Center for Disease Control and Prevention, ⁶CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences

A protocol to evaluate antigen-specific T-cell responses in the immunoprivileged organs of the Ifnar1^-/- murine model for the Zika virus (ZIKV) infection is described. This method is pivotal for investigating the cellular mechanisms of the protection and immunopathogenesis of ZIKV vaccines and is also valuable for their efficacy evaluation.

Immunology and Infection

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β₂-Microglobulin

Di Zhang^*1,2, Kefang Liu^*3,4, Dan Lu^*2,5, Pengyan Wang^1,2, Qingxu Zhang^1,2, Peipei Liu², Yingze Zhao², Yan Chai⁴, Jianxin Lyu¹, Jianxun Qi⁴, William J. Liu^1,2

¹School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, ²NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, ³Faculty of Health Sciences, University of Macau, ⁴CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, ⁵Savaid Medical School, University of Chinese Academy of Sciences

The protocol describes experimental methods to obtain stable major histocompatibility complex (MHC) class I through potential β₂-microglobulin (β₂m) substitutions from different species. The structural comparison of MHC I stabilized by homologous and heterologous β₂m were investigated.

Immunology and Infection

Construction of Adenoviral Vectors using DNA Assembly Technology

Xiaohui Zou^*1, Yun Zhu^*2, Chenglong Li^1,3, Yali Duan², Linlin Zhang², Xiaojuan Guo¹, Wenzhe Hou¹, Zhengde Xie^2,4, Zhuozhuang Lu^1,5

¹NHC Key Laboratory of Medical Virology and Viral Diseases, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, ²Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Center for Children’s Health, ³Henan Chemical Technician College, ⁴Research Unit of Critical Infection in Childre, Chinese Academy of Medical Sciences, ⁵Chinese Academy of Sciences Joint Research Center for Emerging Infectious Diseases and Biosafety, Chinese Center for Disease Control and Prevention-Wuhan Institute of Virology

In this study, an infectious clone of human adenovirus type 7 (HAdV-7) was constructed, and an E3-deleted HAdV-7 vector system was established by modifying the infectious clone. This strategy used here can be generalized to make gene transfer vectors from other wild-type adenoviruses.