Slicing and Culturing Pig Hearts under Physiological ConditionsQinghui Ou *1, Riham R.E. Abouleisa *1, Xian-Liang Tang 1, Hamzah R. Juhardeen 1, Moustafa H. Meki 2, Jessica M. Miller 2, Guruprasad Giridharan 2, Ayman El-Baz 2, Roberto Bolli 1, Tamer M.A. Mohamed 1,3,4,5,6
1Institute of Molecular Cardiology, Department of Medicine, University of Louisville, 2Department of Bioengineering, University of Louisville, 3Diabetes and Obesity Center, Department of Medicine, University of Louisville, 4Department of Pharmacology and Toxicology, University of Louisville, 5Institute of Cardiovascular Sciences, University of Manchester, 6Faculty of Pharmacy, Zagazig University
This protocol describes how to slice and culture heart tissue under physiological conditions for 6 days. This culture system could be used as a platform for testing the efficacy of novel heart failure therapeutics as well as reliable testing of acute cardiotoxicity in a 3D heart model.
