Accedi

Universidad Europea de Madrid

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Fabio Cafini^*1,2, Nguyen Thi Le Thuy^*3, Federico Román⁴, José Prieto⁵, Sarah Dubrac^6,7, Tarek Msadek^6,7, Kazuya Morikawa¹

¹Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, ²Department of Basic Biomedical Science, Universidad Europea de Madrid, ³Human Biology Program, School of Integrative and Global Majors, University of Tsukuba, ⁴Laboratory of Nosocomial Infections, Department of Bacteriology, Centro Nacional de MicrobiologÍa, Instituto de Salud Carlos III, ⁵Division of Microbiology, Department of Medicine, School of Medicine, Universidad Complutense, ⁶Biology of Gram-Positive Pathogens, Department of Microbiology, Institut Pasteur, Paris, France, ⁷ERL3526, CNRS, Paris, France

We describe here three different protocols for the in vitro investigation of conjugation, transduction, and natural transformation in Staphylococcus aureus.

Developmental Biology

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

María José Blanco^*1, Ana I.R. Learte^*1, Miguel A. Marchena¹, Emma Muñoz-Sáez¹, María Antonia Cid¹, Iván Rodríguez-Martín¹, Cristina Sánchez-Camacho^2,3

¹Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, ²Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), ³School of Doctoral Studies and Research, Universidad Europea de Madrid

Here we describe the standard protocol for the detection of β-galactosidase activity in early whole mouse embryos and the method for paraffin sectioning and counterstaining. This is an easy and quick procedure to monitor gene expression during development that can also be applied to tissue sections, organs or cultured cells.