Accedi

Albert Einstein College of Medicine, Yeshiva University

2 ARTICLES PUBLISHED IN JoVE

Biology

Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry

Hao Jiang¹, Lei Feng¹, David Soriano del Amo¹, Ronald D. Seidel III², Florence Marlow³, Peng Wu¹

¹Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, ²Macromolecular Therapeutics Development Facility, Albert Einstein College of Medicine, Yeshiva University, ³Developmental and Molecular Biology, Albert Einstein College of Medicine, Yeshiva University

A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.

Engineering

Plasma-Assisted Molecular Beam Epitaxy Growth of Mg₃N₂ and Zn₃N₂ Thin Films

Peng Wu¹, Thomas Tiedje¹

¹Department of Electrical and Computer Engineering, University of Victoria

This article describes the growth of epitaxial films of Mg₃N₂ and Zn₃N₂ on MgO substrates by plasma-assisted molecular beam epitaxy with N₂ gas as the nitrogen source and optical growth monitoring.