Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 MacrophagesMaruti Nandan Rai 1, Sapan Borah 1, Gaurav Bairwa *1, Sriram Balusu *1,2, Neelima Gorityala 1, Rupinder Kaur 1
1Laboratory of Fungal Pathogenesis, Centre for DNA Fingerprinting and Diagnostics, Andhra Pradesh, India, 2Current location: VIB Department for Molecular Biomedical Research, UGent, Fiers-Schell-Van Montagu Building, Technologiepark 927, B-9052 Ghent (Zwijnaarde), Belgium
The current article outlines a protocol to establish an in vitro cell culture model system to study the interaction of a facultative intracellular human fungal pathogen Candida glabrata with human macrophages which will be a useful tool to advance our knowledge of fungal virulence mechanisms.
