Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel StrokeLora Talley Watts 1, Wei Zheng 1, R. Justin Garling 2, Victoria C. Frohlich 3, James Donald Lechleiter 1
1Department of Cellular and Structural Biology, The University of Texas Health Science Center San Antonio, 2School of Medicine, The University of Texas Health Science Center San Antonio, 3Cell & Tissue Imaging Center, St. Jude Children’s Research Hospital
Here, we describe a semi-invasive optical microscopy approach for the induction of a Rose Bengal photothrombotic clot in the somatosensory cortex of a mouse in vivo. The technical aspects of the imaging procedure are described from induction of a photothrombotic event to application and data collection.
