Accedi

University of Clermont-Ferrand

1 ARTICLES PUBLISHED IN JoVE

Biology

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

Benjamin Bertin¹, Yoan Renaud¹, Rajaguru Aradhya², Krzysztof Jagla¹, Guillaume Junion¹

¹University of Clermont-Ferrand, ²Memorial Sloan Kettering Cancer Center

Translating Ribosome Affinity Purification (TRAP) is able to capture cell-type-specific translation of mRNA. Here we report the first TRAP protocol dedicated to isolation of mRNA in rare cell populations of Drosophila embryos.