University of Clermont-Ferrand

1 ARTICLES PUBLISHED IN JoVE

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Biology

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos
Benjamin Bertin 1, Yoan Renaud 1, Rajaguru Aradhya 2, Krzysztof Jagla 1, Guillaume Junion 1
1University of Clermont-Ferrand, 2Memorial Sloan Kettering Cancer Center

Translating Ribosome Affinity Purification (TRAP) is able to capture cell-type-specific translation of mRNA. Here we report the first TRAP protocol dedicated to isolation of mRNA in rare cell populations of Drosophila embryos.

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