Accedi

Indiana University of School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

Nathan J. Alves^1,2, Kendrick B. Turner¹, Scott A. Walper¹

¹Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, ²Department of Emergency Medicine, Indiana University of School of Medicine

A protocol for the production, purification, and use of enzyme packaged outer membrane vesicles (OMV) providing for enhanced enzyme stability for implementation across diverse applications is presented.

Medicine

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization

Ziqian Zeng^1,2, Tanmaye Nallan Chakravarthula^1,2, Nathan J. Alves^1,2

¹Emergency Medicine Department, Indiana University School of Medicine, ²Weldon School of Biomedical Engineering, Purdue University

Fibrin is responsible for clot formation during hemostasis and thrombosis. Turbidity assays and thromboelastograhy (TEG) can be utilized as synergistic tools that provide complementary assessment of a clot. These two techniques together can give more insight into how clotting conditions affect fibrin clot formation.