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Charité-Universitätsmedizin

6 ARTICLES PUBLISHED IN JoVE

Medicine

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Odilo Engel¹, Sabine Kolodziej¹, Ulrich Dirnagl¹, Vincent Prinz¹

¹Department for Experimental Neurology, Center for Stroke Research Berlin, Charité Universitätsmedizin

Filamentous occlusion of the Middle cerebral artery is a common model for studying ischemic stroke in mice.

Education

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Robert Szulcek¹, Harm Jan Bogaard¹, Geerten P. van Nieuw Amerongen²

¹Department of Pulmonary Diseases, Institute for Cardiovascular Research, VU University Medical Center, ²Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center

This protocol reviews Electric Cell-substrate Impedance Sensing, a method to record and analyze the impedance spectrum of adherent cells for the quantification of cell attachment, proliferation, motility, and cellular responses to pharmacological and toxic stimuli. Detection of endothelial permeability and assessment of cell-cell and cell-substrate contacts are emphasized.

Neuroscience

Lateral Chronic Cranial Window Preparation Enables In Vivo Observation Following Distal Middle Cerebral Artery Occlusion in Mice

Simon H. Bayerl¹, Melina Nieminen-Kelhä¹, Thomas Broggini², Peter Vajkoczy¹, Vincent Prinz¹

¹Department of Neurosurgery and Center for Stroke-research Berlin (CSB), Charité-Universitätsmedizin, ²Department of Physics, University of California San Diego

Surgical occlusion of a distal middle cerebral artery branch (MCAo) is a frequently used model in experimental stroke research. This manuscript describes the basic technique of permanent MCAo, combined with the insertion of a lateral cranial window, which offers the opportunity for longitudinal intravital microscopy in mice.

Immunology and Infection

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

Xue D. Manz¹, Hugo J. Albers^2,3, Petr Symersky⁴, Jurjan Aman¹, Andries D. van der Meer³, Harm Jan Bogaard¹, Robert Szulcek^1,5,6

¹Department of Pulmonary Diseases, Amsterdam UMC, VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), ²BIOS Lab-on-a-Chip group, University of Twente, ³Applied Stem Cell Technologies Group, University of Twente, ⁴Department of Cardio-thoracic Surgery, Amsterdam UMC, VU University Medical Center, ⁵Institute of Physiology, Charité-Universitätsmedizin, ⁶German Heart Center

We present an in vitro vascular disease model to investigate whole blood interactions with patient-derived endothelium. This system allows the study of thrombogenic properties of primary endothelial cells under various circumstances. The method is especially suited to evaluate in situ thrombogenicity and anticoagulation therapy during different phases of coagulation.

Medicine

Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation And Continuous Telemetry

Wen-Jia Liu^*1,2, Lisa Ernst^*2, Benedict Doorschodt², Jan Bednarsch¹, Felix Becker³, Richi Nakatake², Yuki Masano², Ulf Peter Neumann^1,4, Sven Arke Lang¹, Peter Boor⁵, Isabella Lurje⁶, Georg Lurje^1,6, René Tolba², Zoltan Czigany^1,2

¹Department of Surgery and Transplantation, Faculty of Medicine, University Hospital RWTH Aachen, ²Institute for Laboratory Animal Science, Faculty of Medicine, University Hospital RWTH Aachen, ³Department of General-, Visceral-, and Transplantation Surgery, University Hospital Muenster, ⁴Department of Surgery, Maastricht University Medical Centre (MUMC), ⁵Institute of Pathology, Faculty of Medicine, University Hospital RWTH Aachen, ⁶Department of Surgery, Campus Charité Mitte | Campus Virchow-Klinikum, Charité-Universitätsmedizin

Large animal models play an essential role in preclinical transplantation research. Due to its similarities to the clinical setup, the porcine model of orthotopic kidney auto-transplantation described in this article provides an excellent in vivo setting for the testing of organ preservation techniques and therapeutic interventions.

Neuroscience

Endovascular Perforation Model for Subarachnoid Hemorrhage Combined with Magnetic Resonance Imaging (MRI)

Shuheng Liu^*1, Katharina Tielking^*1, Dario von Wedel¹, Melina Nieminen-Kelhä¹, Susanne Mueller^2,3, Philipp Boehm-Sturm^2,3, Peter Vajkoczy¹, Ran Xu¹

¹Department of Neurosurgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, and Humboldt-Universität zu Berlin, and Berlin Institute of Health, ²Department of Experimental Neurology and Center for Stroke Research Berlin, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ³NeuroCure Cluster of Excellence and Charité Core Facility 7T Experimental MRIs, Charité - Universitätsmedizin Berlin

Here we present a standardized SAH mouse model, induced by endovascular filament perforation, combined with magnetic resonance imaging (MRI) 24 h after operation to ensure the correct bleeding site and exclude other relevant intracranial pathologies.