Accedi

University Hospital, Geneva

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

Archana Vijaya Kumar^1,2, Carmen Donate², Beat A. Imhof¹, Thomas Matthes²

¹Department of Pathology and Immunology, University of Geneva, ²Hematology Service, University Hospital, Geneva

Mantle cell lymphoma (MCL) is a difficult to treat B cell disorder and it is equally difficult to establish a xenograft mouse model of primary MCL to study and develop therapeutics. Here, we describe the successful establishment of MCL xenografts in mice to help understand its underlying biology.

Immunology and Infection

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Patricia Ropraz¹, Beat A. Imhof², Thomas Matthes¹, Bernhard Wehrle-Haller³, Adama Sidibé³

¹Hematology service, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, ²Department of Pathology and Immunology, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, ³Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva

Here, we present an integrated protocol that measures monocyte subpopulation trafficking under flow in vitro by use of specific surface markers and confocal fluorescence microscopy. This protocol can be used to explore sequential recruitment steps as well as to profile other leukocyte subtypes using other specific surface markers.