Accedi

Kent State University at Stark

2 ARTICLES PUBLISHED IN JoVE

Biology

The Ingestion of Fluorescent, Magnetic Nanoparticles for Determining Fluid-uptake Abilities in Insects

Matthew S. Lehnert¹, Kristen E. Reiter¹, Andrew Bennett¹, Patrick D. Gerard², Qi-Huo Wei³, Miranda Byler¹, Huan Yan³, Wah-Keat Lee⁴

¹Department of Biological Sciences, Kent State University at Stark, ²Department of Mathematical Sciences, Clemson University, ³Liquid Crystal Institute, Kent State University, ⁴Brookhaven National Laboratory

Fluid-feeding insects have the ability to acquire minute quantities of liquids from porous surfaces. This protocol describes a method to directly determine the ability for insects to ingest liquids from porous surfaces using feeding solutions with fluorescent, magnetic nanoparticles.

Biology

IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae

Victoria A. Ingham¹, Andrew Bennett², Duo Peng³, Simon C. Wagstaff², Hilary Ranson¹

¹Vector Biology, Liverpool School of Tropical Medicine, ²Research Computing Unit, Liverpool School of Tropical Medicine, ³Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health

IR-TEx explores insecticide resistance-related transcriptional profiles in the species Anopheles gambiae. Provided here are full instructions for using the application, modifications for exploring multiple transcriptomic datasets, and using the framework to build an interactive database for collections of transcriptomic data from any organism, generated in any platform.