Accedi

Agency for Science Technology and Research

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components

Sau Yin Chin^1,2, Yukkee Cheung Poh², Anne-Céline Kohler², Samuel K. Sia²

¹Molecular Engineering Laboratory, Biomedical Sciences Institute, Agency for Science Technology and Research, ²Department of Biomedical Engineering, Columbia University

An additive manufacturing strategy for processing UV-crosslinkable hydrogels has been developed. This strategy allows for the layer-by-layer assembly of microfabricated hydrogel structures as well as the assembly of independent components, yielding integrated devices containing moving components that are responsive to magnetic actuation.

Genetics

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Kaiwen Ivy Liu¹, Norfala-Aliah Binte Sutrisnoh¹, Yuanming Wang^1,2, Meng How Tan^1,2

¹Genome Institute of Singapore, Agency for Science Technology and Research, ²School of Chemical and Biomedical Engineering, Nanyang Technological University

CRISPR-Cas is a powerful technology to engineer the complex genomes of plants and animals. Here, we detail a protocol to efficiently edit the human genome using different Cas endonucleases. We highlight important considerations and design parameters to optimize editing efficiency.