Accedi

Center for Information and Neural Networks

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

Naoki Ihara¹, Yuji Ikegaya^1,2, Haruki Takeuchi^1,3

¹Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, ²Center for Information and Neural Networks, National Institute of Information and Communications Technology, ³PRESTO, Japan Science and Technology Agency (JST)

Olfactory sensory neurons express a wide variety of axon-sorting molecules to establish proper neural circuitry. This protocol describes an immunohistochemical staining method to visualize combinatorial expressions of axon-sorting molecules at the axon termini of olfactory sensory neurons.

Neuroscience

Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat

Yu Shikano¹, Takuya Sasaki¹, Yuji Ikegaya^1,2

¹Graduate School of Pharmaceutical Sciences, The University of Tokyo, ²Center for Information and Neural Networks

This study introduces a method for the simultaneous recording of local field potentials in the brain, electrocardiograms, electromyograms, and breathing signals of a freely moving rat. This technique, which reduces experimental costs and simplifies data analysis, will contribute to the understanding of the interactions between the brain and peripheral organs.