Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary ElectrophoresisMarkus Nachbar 1, Jorrit Maul 1, Matthias Stein 1, Hermann Wätzig 1
1Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig
This protocol combines the characterization of a protein sample by capillary gel electrophoresis and a fast-binding screening for charged ligands by affinity capillary electrophoresis. It is recommended for proteins with a flexible structure, such as intrinsically disordered proteins, to determine any differences in binding for different conformers.
