TU Braunschweig

1 ARTICLES PUBLISHED IN JoVE

image

Biochemistry

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
Markus Nachbar 1, Jorrit Maul 1, Matthias Stein 1, Hermann Wätzig 1
1Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig

This protocol combines the characterization of a protein sample by capillary gel electrophoresis and a fast-binding screening for charged ligands by affinity capillary electrophoresis. It is recommended for proteins with a flexible structure, such as intrinsically disordered proteins, to determine any differences in binding for different conformers.

JoVE Logo

개인 정보 보호

이용 약관

정책

연구

교육

JoVE 소개

Copyright © 2025 MyJoVE Corporation. 판권 소유