Accedi

Université de Haute-Alsace

2 ARTICLES PUBLISHED IN JoVE

Chemistry

Photogeneration of N-Heterocyclic Carbenes: Application in Photoinduced Ring-Opening Metathesis Polymerization

Julien Pinaud¹, Emeline Placet¹, Patrick Lacroix-Desmazes¹, Thi Kim Hoang Trinh^2,3, Jean Pierre Malval^2,3, Abraham Chemtob^2,3, Loïc Pichavant⁴, Valérie Héroguez⁴

¹ICGM, Université de Montpellier, CNRS, ENSCM, ²Institut de Science des Matériaux de Mulhouse (IS2M UMR 7361 CNRS), Université de Haute-Alsace, ³Université de Strasbourg, ⁴Laboratoire de Chimie des Polymères Organiques (LCPO UMR 5629 ENSCBP), Université de Bordeaux

We describe a protocol to photogenerate N-heterocyclic carbenes (NHCs) by UV irradiation of a 2-isopropylthioxanthone/imidazolium tetraphenylborate salt system. Methods to characterize the photoreleased NHC and elucidate the photochemical mechanism are proposed. The protocols for ring-opening metathesis photopolymerization in solution and miniemulsion illustrate the potential of this 2-component NHC photogenerating system.

Biochemistry

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Kyle G. Kroeck^1,2, Weihua Qiu^1,2, Claudio Catalano^1,2, Thi Kim Hoang Trinh^1,2, Youzhong Guo^1,2

¹Department of Medicinal Chemistry, School of Pharmacy, Virginia Commonwealth University, ²Institute for Structural Biology, Drug Discovery and Development, School of Pharmacy, Virginia Commonwealth University

Presented here is a protocol for the determination of oligomeric state of membrane proteins that utilizes a native cell membrane nanoparticle system in conjunction with electron microscopy.