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École Normale Supèrieure, Institut de Biologie de l'ENS (IBENS), Institut national de la santè et de la recherche mèdicale (INSERM), Centre national de la recherche scientifique (CNRS), École Normale Supèrieure, PSL Research University

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Dissection of Local Ca²⁺ Signals in Cultured Cells by Membrane-targeted Ca²⁺ Indicators

Hiroko Bannai^1,2, Matsumi Hirose², Fumihiro Niwa^2,3, Katsuhiko Mikoshiba²

¹Japan Science and Technology Agency, PRESTO, ²Laboratory for Developmental Neurobiology, RIKEN Center for Brain Science, ³École Normale Supèrieure, Institut de Biologie de l'ENS (IBENS), Institut national de la santè et de la recherche mèdicale (INSERM), Centre national de la recherche scientifique (CNRS), École Normale Supèrieure, PSL Research University

Here we present a protocol for Ca²⁺ imaging in neurons and glial cells, which enables the dissection of Ca²⁺ signals at subcellular resolution. This process is applicable to all cell types that allow the expression of genetically encoded Ca²⁺ indicators.