Accedi

Instituto de Ciências Biomédicas Abel Salazar

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins

Carlos Flores^1,2,3, Paula Tamagnini^1,2,4

¹Bioengineering and Synthetic Microbiology Group, Instituto de Investigação e Inovação em Saude, Universidade do Porto, ²Instituto de Biologia Celular e Molecular, Universidade do Porto, ³Instituto de Ciências Biomédicas Abel Salazar, ⁴Departamento de Biologia, Faculdade de Ciências, Universidade do Porto

Here, protocols for the isolation of cyanobacterial released carbohydrate polymers and isolation of their exoproteomes are described. Both procedures embody key steps to obtain polymers or proteins with high purity degrees that can be used for further analysis or applications. They can also be easily adapted according to specific user needs.