Accedi

Arizona Alzheimer's Consortium

1 ARTICLES PUBLISHED IN JoVE

Genetics

Identification of Circular RNAs using RNA Sequencing

Shobana Sekar^1,2, Philipp Geiger^1,2, Lori Cuyugan^1,2, Annalee Boyle^1,2, Geidy Serrano^2,3, Thomas G. Beach^2,3, Winnie S. Liang^1,2

¹Translational Genomic Research Institute, ²Arizona Alzheimer's Consortium, ³Banner Sun Health Research Institute

Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented here.