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National Quemoy University

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
Yu-Jen Chen 1,2, Hui-Yu Liu 2, Yun-Tsui Chang 2, Ying-Hung Cheng 2, Harry J. Mersmann 2, Wen-Hung Kuo 3, Shih-Torng Ding 1,2
1Institute of Biotechnology, National Taiwan University, 2Department of Animal Science and Technology, National Taiwan University, 3Department of Surgery, National Taiwan University Hospital and College of Medicine

This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies.

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Medicine

Integration of Brain Tissue Saturation Monitoring in Cardiopulmonary Exercise Testing in Patients with Heart Failure
Shu-Chun Huang 1,2,3, Carl PC Chen 1,3, Tieh-Cheng Fu 4, Yu-Jen Chen 1,5,6
1Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Linkou, 2Healthy Aging Research Center, Chang Gung University, 3School of Medicine, College of Medicine, Chang Gung University, 4Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Keelung, 5National Quemoy University, 6Kinmen Hospital, Ministry of Health and Welfare

This protocol integrated near-infrared spectroscopy into conventional cardiopulmonary exercise testing to identify the involvement of the cerebral hemodynamic response in exercise intolerance in patients with heart failure.

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