Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow CytometryMukul Girotra *1,2, Anne-Christine Thierry 3, Alexandre Harari 1,3, George Coukos 1, Olaia Naveiras 2,4, Nicola Vannini *1
1Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne, University of Lausanne, 2Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), 3Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois, 4Hematology Service, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV)
Here we describe a reliable method to measure mitochondrial mass and membrane potential in ex vivo cultured hematopoietic stem cells and T cells.