Accedi

Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg

1 ARTICLES PUBLISHED IN JoVE

Medicine

Investigating Intestinal Barrier Breakdown in Living Organoids

Marco Bardenbacher¹, Barbara Ruder², Natalie Britzen-Laurent¹, Elisabeth Naschberger¹, Christoph Becker², Ralph Palmisano³, Michael Stürzl^*1, Philipp Tripal^*1,3

¹Division of Molecular and Experimental Surgery, Department of Surgery, Translational Research Center, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, ²Department of Medicine 1, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, ³Optical Imaging Centre Erlangen (OICE), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg

Here we describe a technique to quantify the barrier integrity of small intestinal organoids. The fact that the method is based on living organoids enables the sequential investigation of different barrier integrity modulating substances or combinations thereof in a time-resolved manner.