An Electrochemiluminescence-Based Assay for MeCP2 Protein VariantsHannes Steinkellner 1, Alexander V. Beribisky 1, Philip Mausberg 1, John Christodoulou 2, Barbara Scheiber-Mojdehkar 3, Anna Huber 1, Victoria Sarne 1, Franco Laccone 1
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), 2Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna
The electrochemiluminescence immunoassay (ECLIA) is a novel approach for quantitative detection of endogenous and exogenously applied MeCP2 protein variants, which produces highly quantitative, accurate and reproducible measurements with low intra- and inter-assay error over a wide working range. Here, the protocol for the MeCP2-ECLIA in a 96-well format is described.
