Accedi

Penn State Hershey Cancer Institute

3 ARTICLES PUBLISHED IN JoVE

Medicine

Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments

Yongjian Zhao^1,2,3, Qiang Wang^1,2,3, Shufen Liu^1,2,3, Yongjun Wang^1,2,3,4, Bing Shu^1,2,3, Dongfeng Zhao^1,2,3

¹Longhua Hospital, Shanghai University of Traditional Chinese Medicine, ²Spine Institute, Shanghai University of Traditional Chinese Medicine, ³Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education, ⁴School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine

This article provides a detailed protocol for preparing a working solution of Gushukang granules for animal studies and GSK granule containing serum for in vitro experiments. This protocol can be applied to pharmacological investigations of herbal medicines as well as prescriptions for both in vivo and in vitro experiments.

Bioengineering

Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library

Ying Zhao^*1, Qiang Wang^*1, An Hong¹, Xiaojia Chen¹

¹Institute of Biomedicine & Department of Cell Biology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine

Herein, we present a detailed protocol for screening small peptides that bind to FGFR2 using a phage display peptide library. We further analyze the affinity of the selected peptides toward FGFR2 in vitro and its ability to suppress cell proliferation.

Developmental Biology

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Aflah Hanafiah¹, Zhuangzhuang Geng¹, Qiang Wang¹, Zhonghua Gao¹

¹Departments of Biochemistry and Molecular Biology, Penn State College of Medicine, Penn State Hershey Cancer Institute

We describe the procedure for the in vitro differentiation of mouse embryonic stem cells into neuronal cells using the hanging drop method. Furthermore, we perform a comprehensive phenotypic analysis through RT-qPCR, immunofluorescence, RNA-seq, and flow cytometry.