Accedi

Aix Marseille Univ, CNRS, INP

2 ARTICLES PUBLISHED IN JoVE

Genetics

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Manon Torres¹, Denis Becquet¹, Séverine Guillen¹, Bénédicte Boyer¹, Mathias Moreno¹, Marie-Pierre Blanchard², Jean-Louis Franc¹, Anne-Marie François-Bellan¹

¹CNRS, CRN2M-UMR7286, Faculté de Médecine Nord, Aix-Marseille Université, ²Plate-forme d'imagerie MRI, UMS3426, CNRS 141, Institut de Génétique Humaine

This RNA pull-down method allows identifying the RNA targets of a long non-coding RNA (lncRNA). Based on the hybridization of home-made, designed anti-sense DNA oligonucleotide probes specific to this lncRNA in an appropriately fixed tissue or cell line, it efficiently allows the capture of all RNA targets of the lncRNA.

Genetics

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Audrey Jacq¹, Denis Becquet¹, Séverine Guillen¹, Bénédicte Boyer¹, Maria-Montserrat Bello-Goutierrez¹, Jean-Louis Franc^*1, Anne-Marie François-Bellan^*1

¹Faculté des Sciences Médicales et Paramédicales, Aix Marseille Univ, CNRS, INP

An easy-to-use RNA pull-down protocol is designed for the identification of RNAs engaged in direct RNA/RNA interaction with a long non-coding RNA. The protocol uses psoralen as a fixative to cross-link only RNA/RNA interactions and provides the whole direct RNA interactome of a long non-coding RNA when coupled with RNA sequencing.