Organotypic Slice Cultures as Preclinical Models of Tumor Microenvironment in Primary Pancreatic Cancer and MetastasisRüdiger Braun 1, Olha Lapshyna 1, Susanne Eckelmann 2,3, Kim Honselmann 1, Louisa Bolm 1, Meike ten Winkel 1, Steffen Deichmann 1, Oliver Schilling 4, Charli Kruse 2,3, Tobias Keck 1, Ulrich Wellner 1, Peter Bronsert 4,5,6, Matthias Brandenburger 3
1Department of Surgery, University Medical Center Schleswig-Holstein, 2Institute of Medical and Marine Biotechnology, University of Lübeck, 3Fraunhofer Research and Development Center for Marine and Cellular Biotechnology, 4Institute for Surgical Pathology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 5Tumorbank Comprehensive Cancer Center Freiburg, Medical Center - University of Freiburg, 6Core Facility Histopathology and Digital Pathology Freiburg, Medical Center - University of Freiburg
This protocol describes the preparation of organotypic slice cultures (OTSCs). This technique facilitates the ex vivo cultivation of intact multicellular tissue. OTSCs can be used immediately to test for their respective response to drugs in a multicellular environment.
