Accedi

Third Affiliated Hospital, Beijing University of Chinese Medicine

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Generation of Monoclonal Antibodies Against Natural Products

Yue Zhang¹, Peng Cao², Fang Lu³, Xin Yan⁴, Bingqian Jiang³, Jinjun Cheng³, Huihua Qu⁵

¹School of Life Science, Beijing University of Chinese Medicine, ²Third Affiliated Hospital, Beijing University of Chinese Medicine, ³School of Basic Medical Sciences, Beijing University of Chinese Medicine, ⁴School of Chinese Materia Medica, Beijing University of Chinese Medicine, ⁵Center of Scientific Experiment, Beijing University of Chinese Medicine

This article provides a detailed protocol for the preparation and evaluation of monoclonal antibodies against natural products for use in various immunoassays. This procedure includes immunization, cell fusion, indirect competitive ELISA for positive clone screening, and monoclonal hybridoma preparation. The specifications for antibody characterization using MALDI-TOF-MS and ELISA analyses are also provided.

Biochemistry

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Yue Zhang^*1, Peng Cao^*2, Fang Lu¹, Jinjun Cheng³, Huihua Qu⁴

¹School of Life Science, Beijing University of Chinese Medicine, ²Third Affiliated Hospital, Beijing University of Chinese Medicine, ³National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, ⁴Center of Scientific Experiment, Beijing University of Chinese Medicine

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds