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Skåne University Hospital

2 ARTICLES PUBLISHED IN JoVE

Medicine

Robotized Testing of Camera Positions to Determine Ideal Configuration for Stereo 3D Visualization of Open-Heart Surgery

Maj Stenmark^1,2, Edin Omerbašić^1,2, Måns Magnusson¹, Sanna Nordberg³, Matilda Dahlström³, Phan-Kiet Tran¹

¹Cardiac Surgery Unit, Children’s Heart Centre, Skåne University Hospital, ²Department of Clinical Sciences, Lund University, ³Student at Lund University

The human depth perception of 3D stereo videos depends on the camera separation, point of convergence, distance to, and familiarity of the object. This paper presents a robotized method for rapid and reliable test data collection during live open-heart surgery to determine the ideal camera configuration.

Medicine

Automated Vibratome Sectioning of Agarose-Embedded Lung Tissue for Multiplex Fluorescence Imaging

Qi Wang^1,2,3, Nicholas B. Bechet^1,2,3, Sandra Lindstedt^1,2,3,4

¹Wallenberg Centre for Molecular Medicine, Lund University, ²Department of Clinical Sciences, Lund University, ³Lund Stem Cell Centre, Lund University, ⁴Department of Cardiothoracic Surgery and Transplantation, Skåne University Hospital

We have developed a tissue-processing technique utilizing a vibratome and agarose-embedded lung tissue to generate lung sections, thereby permitting the acquisition of high-resolution images of lung architecture. We employed immunofluorescence staining to observe spatial protein expression using specific lung structural markers.