Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live CellsKatherina Hemmen *1, Susobhan Choudhury *1, Mike Friedrich 1, Johannes Balkenhol 1, Felix Knote 1, Martin J. Lohse 2, Katrin G. Heinze 1
1Rudolf Virchow Center for Integrative and Translation Bioimaging, Julius-Maximilian University Wuerzburg, 2Max Delbrück Center for Molecular Medicine, Berlin
We present an experimental protocol and data analysis workflow to perform live cell dual-color fluorescence cross correlation spectroscopy (FCCS) combined with Förster Resonance Energy transfer (FRET) to study membrane receptor dynamics in live cells using modern fluorescence labeling techniques.
