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Julius-Maximilian University Wuerzburg

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Katherina Hemmen^*1, Susobhan Choudhury^*1, Mike Friedrich¹, Johannes Balkenhol¹, Felix Knote¹, Martin J. Lohse², Katrin G. Heinze¹

¹Rudolf Virchow Center for Integrative and Translation Bioimaging, Julius-Maximilian University Wuerzburg, ²Max Delbrück Center for Molecular Medicine, Berlin

We present an experimental protocol and data analysis workflow to perform live cell dual-color fluorescence cross correlation spectroscopy (FCCS) combined with Förster Resonance Energy transfer (FRET) to study membrane receptor dynamics in live cells using modern fluorescence labeling techniques.