Accedi

Sinai Health Systems

3 ARTICLES PUBLISHED IN JoVE

Medicine

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

Kaiyue Zhang¹, Chen Wang¹, Ran Wang², Shang Chen¹, Zongjin Li¹

¹Nankai University School of Medicine, ²State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University

This protocol describes the establishment of a tumor-bearing mouse model to monitor tumor progression and angiogenesis in real-time by dual bioluminescence imaging.

Medicine

Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes

Yun Liu^*1, Yin Liang^*1, Mengxue Wang^*1, Chen Wang¹, Heng Wei², Keiji Naruse¹, Ken Takahashi¹

¹Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, ²Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

We present a model of ischemic heart disease using cardiomyocytes derived from human induced pluripotent stem cells, together with a method for quantitative evaluation of tissue damage caused by ischemia. This model can provide a useful platform for drug screening and further research on ischemic heart disease.

Immunology and Infection

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Marieke de Korte^1,2, Armand Keating^1,2,4, Chen Wang^1,3

¹Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, ²Krembil Research Institute, Toronto Western Hospital, University Health Network, ³Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital, Sinai Health Systems, ⁴Medical Oncology and Hematology, Princess Margaret Cancer Centre

This is a step-by-step guide for using a commercially available rotary cell culture system to culture lymphocytes in simulated microgravity using specialized disposable culture vessels. This culturing method may be applied to any suspension-type cell culture.