Accedi

William Beaumont School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

Isolation of Primary Mouse Retinal Pigmented Epithelium Cells

Ryan Tomaszewski^1,2, Pragya Rajpurohit³, Mei Cheng^1,2, Amany Tawfik^1,2,3

¹Eye Research Institute, Oakland University, ²Eye Research Center (OUWB)/ERC, William Beaumont School of Medicine, ³Oral Biology and Diagnostic Sciences, Dental College of Georgia, Augusta University

This manuscript describes a simplified protocol for the isolation of retinal pigmented epithelium (RPE) cells from mouse eyes in a stepwise manner. The protocol includes the enucleation and dissection of mouse eyes, followed by the isolation, seeding, and culturing of RPE cells.

Biology

Isolation of Primary Mouse Retinal Glial Müller Cells

Ryan Tomaszewski¹, Mohamed S. Gad¹, Paul Negoita¹, Rana Abdelkarim¹, Amany Tawfik^1,2

¹Eye Research Institute, Oakland University, ²Eye Research Center (OUWB)/ERC, William Beaumont School of Medicine

This manuscript describes a detailed protocol for isolating retinal glial Müller cells from mouse eyes. The protocol starts with enucleation and dissection of mouse eyes, followed by isolation, seeding, and culturing of Müller cells.