Accedi

University of New Mexico School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Elizabeth M. Bailey¹, Emanuel Salazar-Cavazos¹, Rachel M. Grattan¹, Michael J. Wester², David J. Schodt², Julian A. Rojo¹, Keith A. Lidke^2,3, Diane S. Lidke^1,3

¹Department of Pathology, University of New Mexico School of Medicine, ²Department of Physics and Astronomy, University of New Mexico, ³Comprehensive Cancer Center, University of New Mexico Health Sciences Center

The present protocol describes sample preparation and data analysis to quantify protein phosphorylation using an improved single-molecule pull-down (SiMPull) assay.

Neuroscience

Combining Imaging and Electrophysiology to Visualize and Record Spreading Depolarizations in Mice

Brandi R. Hess^*1, Devon S. Hatcher^*1, Johann Pacheco¹, Myah K. McDonald¹, Natalie J. Pinkowski¹, Raycarlo Laboy-Ramirez¹, Z. Matthew Situ¹, Taha F. Abdeljawad¹, Carissa J. Mehos¹, Russell A. Morton¹

¹Department of Neurosciences and The Center for Brain Recovery and Repair, University of New Mexico School of Medicine

This protocol demonstrates the combination of imaging and electrophysiology to reliably detect spreading depolarizations in adult mice following a mild traumatic brain injury.