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University of New Mexico School of Medicine

2 ARTICLES PUBLISHED IN JoVE

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Biochemistry

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
Elizabeth M. Bailey 1, Emanuel Salazar-Cavazos 1, Rachel M. Grattan 1, Michael J. Wester 2, David J. Schodt 2, Julian A. Rojo 1, Keith A. Lidke 2,3, Diane S. Lidke 1,3
1Department of Pathology, University of New Mexico School of Medicine, 2Department of Physics and Astronomy, University of New Mexico, 3Comprehensive Cancer Center, University of New Mexico Health Sciences Center

The present protocol describes sample preparation and data analysis to quantify protein phosphorylation using an improved single-molecule pull-down (SiMPull) assay.

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Neuroscience

Combining Imaging and Electrophysiology to Visualize and Record Spreading Depolarizations in Mice
Brandi R. Hess *1, Devon S. Hatcher *1, Johann Pacheco 1, Myah K. McDonald 1, Natalie J. Pinkowski 1, Raycarlo Laboy-Ramirez 1, Z. Matthew Situ 1, Taha F. Abdeljawad 1, Carissa J. Mehos 1, Russell A. Morton 1
1Department of Neurosciences and The Center for Brain Recovery and Repair, University of New Mexico School of Medicine

This protocol demonstrates the combination of imaging and electrophysiology to reliably detect spreading depolarizations in adult mice following a mild traumatic brain injury.

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