Accedi

Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Eva Dovjak¹, Mario Mairhofer¹, Claudia Wöß¹, Jingjing Qi¹, Dorothy N. Y. Fan², Clemens A. Schmitt^1,2,3, Yong Yu¹

¹Johannes Kepler University Linz, ²Charité - University Medicine Berlin, ³Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association

Here we present a flow cytometry-based method for visualization and quantification of multiple senescence-associated markers in single cells.

Neuroscience

Quantification of Microglial Engulfment of Synaptic Material Using Flow Cytometry

Bilge Ugursu¹, Susanne A. Wolf^1,2

¹Psychoneuroimmunology, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, ²Experimental Ophthalmology, Charité Universitätsmedizin Berlin

Here we present two protocols to quantify microglial engulfment of vGLUT1-positive synapses and pHRodo Red-labeled crude synaptosomes using flow cytometry.