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Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association

2 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
Eva Dovjak 1, Mario Mairhofer 1, Claudia Wöß 1, Jingjing Qi 1, Dorothy N. Y. Fan 2, Clemens A. Schmitt 1,2,3, Yong Yu 1
1Johannes Kepler University Linz, 2Charité - University Medicine Berlin, 3Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association

Here we present a flow cytometry-based method for visualization and quantification of multiple senescence-associated markers in single cells.

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Neuroscience

Quantification of Microglial Engulfment of Synaptic Material Using Flow Cytometry
Bilge Ugursu 1, Susanne A. Wolf 1,2
1Psychoneuroimmunology, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, 2Experimental Ophthalmology, Charité Universitätsmedizin Berlin

Here we present two protocols to quantify microglial engulfment of vGLUT1-positive synapses and pHRodo Red-labeled crude synaptosomes using flow cytometry.

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