Accedi

University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences

1 ARTICLES PUBLISHED IN JoVE

Genetics

Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

Mathilde Beaufils¹, Amandine Tourel¹, Anne Petiot¹, Nicole B. Halmai², Dave J. Segal², John Rendu¹, Isabelle Marty¹

¹University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, ²Genome Center, University of California, Davis

The protocol describes how to generate knock-out myoblasts using CRISPR/Cas9, starting from the design of guide-RNAs to the cellular cloning and characterization of the knock-out clones.