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Marquette University

6 ARTICLES PUBLISHED IN JoVE

Behavior

An Alternative to the Traditional Cold Pressor Test: The Cold Pressor Arm Wrap

¹Department of Psychology, Marquette University

The cold pressor test is a classic stress induction task used in a wide range of research examining the relationship between stress and cognition, emotion, and behavior. In this article, a practical and versatile alternative to the traditional cold pressor test is presented: the cold pressor arm wrap.

Neuroscience

Diffusion Imaging in the Rat Cervical Spinal Cord

Elizabeth Zakszewski¹, Brian Schmit², Shekar Kurpad¹, Matthew D. Budde¹

¹Department of Neurosurgery, Medical College of Wisconsin, ²Department of Biomedical Engineering, Marquette University

The goal of this protocol is to obtain high-quality diffusion weighted magnetic resonance imaging (DWI) of the rat spinal cord for noninvasive characterization of tissue microstructure. This protocol describes optimizations of the MRI sequence, radiofrequency coil, and analysis methods to enable DWI images free from artifacts.

Engineering

Thermal Measurement Techniques in Analytical Microfluidic Devices

Benyamin Davaji¹, Chung Hoon Lee¹

¹Nanoscale Devices Laboratory, Marquette University

Here, we present three protocols for thermal measurements in microfluidic devices.

Medicine

Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats

Kathleen M. Lukaszewicz¹, Matthew J. Durand², Jessica R.C. Priestley³, James R. Schmidt⁴, L. Adrienne Allen⁵, Aron M. Geurts³, Julian H. Lombard³

¹Department of Physical Therapy, Marquette University, ²Medical College of Wisconsin, ³Department of Physiology, Medical College of Wisconsin, ⁴Graduate Programs of Nurse Anesthesia, Texas Wesleyan University, ⁵Office of Research, Medical College of Wisconsin

This manuscript describes in vitro video microscopy protocols for evaluating vascular function in rat cerebral resistance arteries. The manuscript also describes techniques for evaluating microvessel density with fluorescently labeled lectin and tissue perfusion using Laser Doppler Flowmetry.

A Collection Of Novel Techniques to Study Diabetes and Obesity

Sukanya Lodh¹

¹Biology Department, Marquette University

A Collection Of Novel Techniques to Study Diabetes and Obesity

Biochemistry

Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader

Jacob T. DeRousse^1,2,3, Chris Dockendorff^1,2

¹Function Therapeutics, ²Department of Chemistry, Marquette University, ³School of Medicine, Washington University

An improved protocol for a calcium mobilization assay with endothelial cells, used to identify ligands of protease-activated receptors (PARs), has been developed. The new protocol reduces total assay time by 90-120 min and yields reproducible concentration-response curves.