Accedi

University of New England

3 ARTICLES PUBLISHED IN JoVE

Biology

Measurement of Larval Activity in the Drosophila Activity Monitor

Aidan L. McParland¹, Taylor L. Follansbee¹, Geoffrey K. Ganter¹

¹Department of Biology, University of New England

This report describes a method for measuring Drosophila larval activity using the TriKinetics Drosophila Activity Monitor. The device employs infrared beams to detect movements of up to 16 individual animals. Data can be analyzed to represent motion parameters including rates and the positions of the animals within the assay chambers.

Neuroscience

Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue

Jennifer T. Malon¹, Ling Cao¹

¹Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England

The development of neuropathic pain involves pathological changes of spinal cord glial cells. A reliable glial culture system derived from adult spinal cord tissue and designed for studying these cells in vitro is lacking. Therefore, we show here how to establish primary mixed glial cultures from adult mouse spinal cord tissue.

Chemistry

Application of Voltage in Dynamic Light Scattering Particle Size Analysis

Tianyu Ren^*1, Emma J. Roberge^*2, John R. Csoros¹, W. Rudolph Seitz¹, Eva Rose M. Balog³, Jeffrey Mark Halpern²

¹Department of Chemistry, University of New Hampshire, ²Department of Chemical Engineering, University of New Hampshire, ³Department of Chemistry and Physics, University of New England

Here, a protocol to apply voltage to solution during dynamic light scattering particle size measurements with the intent to explore the effect of voltage and temperature changes on polymer aggregation is presented.