Accedi

Ajou University

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Jae H. Lee^1,2, Yong II Cho⁵, Sung S. Choi⁴, Hae-Won Kim^1,2, Churl K. Min³, Sang J. Lee⁵

¹Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, ²Insititute of Tissue Regeneration Engineering, Dankook University, ³Department of Biological Sciences, Ajou University, ⁴Department of Pharmacy, Sahmyook University, ⁵Department of Animal Biotechnology, Sahmyook University

The goal of this protocol is to describe a loss- and gain-of function method that is applicable to identify neogenin as a stage-specific receptor that leads to trophectoderm and inner cell mass differentiation in preimplantation mouse embryos.

Biology

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

Hyunhee Kim¹, Juwon Jeong¹, Changhan Lee¹

¹Department of Biological Sciences, Ajou University

An in vivo physiological model of α-synuclein is required to study and understand the pathogenesis of Parkinson's disease. We describe a method to monitor the cytotoxicity and aggregate formation of α-synuclein using a humanized yeast model.