Christian Medical College

2 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype
Saswati Das 1,2, Raveen Stephen Stallon Illangeswaran 1,2, Smitha Ijee 2,3, Sanjay Kumar 2,3, Shaji Ramachandran Velayudhan 1,2,3, Poonkuzhali Balasubramanian 1,2,3
1Department of Clinical Haematology, Christian Medical College, 2Department of Biotechnology, Thiruvalluvar University, 3Centre for Stem Cell Research, Christian Medical College

High-throughput RNA interference (RNAi) screening using a pool of lentiviral shRNAs can be a tool to detect therapeutically relevant synthetic lethal targets in malignancies. We provide a pooled shRNA screening approach to investigate the epigenetic effectors in acute myeloid leukemia (AML).

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Biology

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay
Elizabeth Vinod 1,2, Ganesh Parasuraman 2, Abel Livingston 3, Solomon Sathishkumar 1, Boopalan Ramasamy 4,5
1Department of Physiology, Christian Medical College, 2Centre for Stem Cell Research (A unit of InStem, Bengaluru), Christian Medical College, 3Department of Orthopedics, Christian Medical College, 4Faculty of Health and Medical Sciences, The University of Adelaide, 5Department of Orthopedics and Trauma, Royal Adelaide Hospital

This protocol details the isolation of chondrocytes, Fibronectin Adhesion Assay-derived Chondroprogenitors (FAA-CPs), and Migratory Chondroprogenitors (MCPs) from human articular cartilage. It covers enzymatic digestion, fibronectin adhesion, and migration-based assays for isolating and characterizing these cells.

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