Accedi

Christian Medical College

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

Saswati Das^1,2, Raveen Stephen Stallon Illangeswaran^1,2, Smitha Ijee^2,3, Sanjay Kumar^2,3, Shaji Ramachandran Velayudhan^1,2,3, Poonkuzhali Balasubramanian^1,2,3

¹Department of Clinical Haematology, Christian Medical College, ²Department of Biotechnology, Thiruvalluvar University, ³Centre for Stem Cell Research, Christian Medical College

High-throughput RNA interference (RNAi) screening using a pool of lentiviral shRNAs can be a tool to detect therapeutically relevant synthetic lethal targets in malignancies. We provide a pooled shRNA screening approach to investigate the epigenetic effectors in acute myeloid leukemia (AML).

Biology

Isolation of Chondrocytes and Chondroprogenitors using Fibronectin Adhesion and Migratory Assay

Elizabeth Vinod^1,2, Ganesh Parasuraman², Abel Livingston³, Solomon Sathishkumar¹, Boopalan Ramasamy^4,5

¹Department of Physiology, Christian Medical College, ²Centre for Stem Cell Research (A unit of InStem, Bengaluru), Christian Medical College, ³Department of Orthopedics, Christian Medical College, ⁴Faculty of Health and Medical Sciences, The University of Adelaide, ⁵Department of Orthopedics and Trauma, Royal Adelaide Hospital

This protocol details the isolation of chondrocytes, Fibronectin Adhesion Assay-derived Chondroprogenitors (FAA-CPs), and Migratory Chondroprogenitors (MCPs) from human articular cartilage. It covers enzymatic digestion, fibronectin adhesion, and migration-based assays for isolating and characterizing these cells.