Finanziamenti forniti dal National Institute on abuso di alcool e l&#39;alcolismo (NIAAA; AA-016967 e AA016967-01S1, TAM), Wayne State University di avvio dei fondi, e la Wayne State University Research Grant Program. Il contenuto è di esclusiva responsabilità degli autori e non rappresenta il punto di vista ufficiale del NIAAA o il National Institutes of Health.

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Nessun conflitto di interessi dichiarati.

Il protocollo presentato qui dimostra come preparare e utilizzare fette coronali del cervello del mouse per gli esperimenti FSCV. Anche se questo metodo è specifico per ottenere e misurare la dinamica della dopamina, neurotrasmettitori altri come l&#39;adenosina, perossido di idrogeno, noradrenalina e serotonina sono stati monitorati in vivo o in vitro con FSCV 3, 8 - 11. FSCV può essere utilizzato per monitorare alcune di queste sostanze neurochimiche altri semplici modifiche della forma ...

<table border="1"><tbody><tr><td> Nome </td><td> Azienda </td><td> Numero CAS </td></tr><tr><td width="274"> Reagente </td><td></td><td></td></tr><tr><td width="274"> Cloruro di potassio </td><td> Pescatore </td><td width="142" valign="top"> 7447407 </td></tr><tr><td width="274"> Cloruro di sodio </td><td> EMD chimici </td><td width="142" valign="top"> 7647145 </td></tr><tr><td width="274"> Cloruro di magnesio </td><td width="194" valign="top"> Pescatore </td><td width="142" valign="top"> 7791186 </td></tr><tr><td width="274"> Cloruro di calcio </td><td width="194" valign="top"> Pescatore </td><td width="142" valign="top"> 10035048 </td></tr><tr><td width="274"> Bicarbonato di sodio </td><td width="194" valign="top"> EMD chimici </td><td width="142" valign="top"> 144558 </td></tr><tr><td width="274"> Fosfato di sodio, dibasico </td><td width="194" valign="top"> EMD chimicas </td><td width="142" valign="top"> 7558794 </td></tr><tr><td width="274"> D-glucosio </td><td width="194" valign="top"> Pescatore </td><td width="142" valign="top"> 50997 </td></tr><tr><td width="274"> Acido ascorbico </td><td width="194" valign="top"> Pescatore </td><td width="142" valign="top"> 50817 </td></tr><tr><td width="274"> Saccarosio </td><td width="194" valign="top"> Pescatore </td><td> 57501 </td></tr><tr><td width="274">&nbsp;</td><td width="194" valign="top">&nbsp;</td><td>&nbsp;</td></tr><tr><td width="274"> Materiale </td><td width="194" valign="top"> Venditore </td><td> Numero di catalogo </td></tr><tr><td width="274"> Fibra di carbonio </td><td> Goodfellow Oakdale, PA </td><td></td></tr><tr><td width="274"> Capillare di vetro </td><td> AM Sistemi Carlsborg WA. </td><td> 602000 </td></tr><tr><td width="274"> In filo d&#39;argento </td><td> A-M Systems Carlsborg WA. </td><td> 787000 </td></tr><tr><td width="274"> Tungsteno elettrodo stimolante </td><td> Una plastica, Roanoke, VA </td><td></td></tr><tr><td width="274"> Filo di platino </td><td></td><td></td></tr><tr><td width="274"> Cavo </td><td> Elettronica scudieri, Cornelius, O </td><td></td></tr><tr><td width="274"> Loctite 404 adesivo istantaneo </td><td> Hankal Corp. Rocky Hill CT. </td><td></td></tr><tr><td width="274"> Lametta </td><td> Mondo precisione Instruments Inc. FL. </td><td></td></tr><tr><td width="274"> BD spinale ago </td><td> BD Medical Systems, Franlin Lake, NJ </td><td> REF 405234 </td></tr><tr><td width="274"> Lama chirurgica </td><td> Piuma di sicurezza rasoio Co. LTD. Giappone </td><td></td></tr><tr><td width="274"></td><td></td><td></td></tr><tr><td width="274"> Software </td><td width="194" valign = &quot;top&quot;&gt; Venditore </td><td> Numero di catalogo </td></tr><tr><td width="274"> TH software </td><td> ESA Inc., Chelmsford, MA </td><td></td></tr><tr><td width="274"> Strumento </td><td width="194" valign="top"> Venditore </td><td> Numero di catalogo </td></tr><tr><td width="274"> Registrazione camera di immersione </td><td> Personalizzati scientifico, Denver, CO </td><td></td></tr><tr><td width="274"> Neorolog stimolo isolatore </td><td> Digitmeter, Hertfordshire, Inghilterra </td><td></td></tr><tr><td width="274"> Controllo automatico della temperatura </td><td> Warner Instrument Corporation </td><td></td></tr><tr><td width="274"> Microscopio (SZX7) </td><td> Olimpo </td><td></td></tr><tr><td width="274"> Microscopio </td><td> Pescatore </td><td></td></tr><tr><td width="274"> Vibratome 3000 sezionamento sistema </td><td> S. Louè, MO. </td><td></td></tr><tr><td width="274"> Perfusione pompa </td><td> Watson Marlow Limited, Falmouth Cornwall - Inghilterra </td><td> H110708 </td></tr><tr><td width="274"> Micropipetta estrattore </td><td> Narishige, Tokyo, Giappone </td><td></td></tr><tr><td width="274"> ChemClamp potenziostato </td><td> Dagan Corporazioni, Minneapolis, MN. </td><td></td></tr></tbody></table>

presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry

La vasta ricerca si è concentrata sul neurotrasmettitore dopamina a causa della sua importanza nel meccanismo d&#39;azione dei farmaci d&#39;abuso (ad esempio la cocaina e anfetamine), il ruolo che gioca in malattie psichiatriche (es. schizofrenia e Attention Deficit Hyperactivity Disorder), e il suo coinvolgimento in degenerativa disturbi come il Parkinson e la malattia di Huntington. In condizioni fisiologiche normali, la dopamina è noto per regolare l&#39;attività locomotoria, conoscenza, apprendimento, influiscono emotivo, e la secrezione ormonale neuroendocrino. Uno dei più grandi densità di neuroni dopaminergici è all&#39;interno del corpo striato, che possono essere suddivisi in due distinte regioni neuroanatomiche conosciuto come il nucleo accumbens e del caudato-putamen. L&#39;obiettivo è quello di illustrare un protocollo generale per la fetta di scansione veloce voltammetria ciclica (FSCV) all&#39;interno del corpo striato mouse. FSCV è una ben definita tecnica elettrochimica fornendo l&#39;opportunità di misurare il rilascio di dopamina e la diffusione in tempo reale discrete cervello regioni. Microelettrodi in fibra di carbonio (diametro di circa 7 micron) sono utilizzati in FSCV per rilevare l&#39;ossidazione della dopamina. Il vantaggio di utilizzare FSCV analitici per rilevare la dopamina è la sua migliore risoluzione temporale di 100 millisecondi e una risoluzione spaziale di meno di dieci micron, fornendo informazioni complementari al microdialisi in vivo.

Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

Tramite scansione veloce voltammetria ciclica per misurare elettricamente evocate dinamiche dopamina presinaptica in fettine di cervello striatale.

Dinamica dopamina presinaptica in fettine di cervello striatale con Fast-scan Voltammetria ciclica

Extensive research has focused on the neurotransmitter dopamine because of its importance in the mechanism of action of drugs of abuse (e.g. cocaine and ...

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21.7K Views.  Wayne State University. Tramite scansione veloce voltammetria ciclica per misurare elettricamente evocate dinamiche dopamina presinaptica in fettine di cervello striatale.